Journal: Journal of Endocrinology

Article Title: Mice deficient in PAPP-A show resistance to the development of diabetic nephropathy

doi: 10.1530/joe-13-0167

Figure Lengend Snippet: Figure 4 Macrophage immunohistochemistry of diabetic WT and Papp-A KO kidney. Macrophage staining for F4/80 (red brown) of kidney sections from WT diabetic (grade 3) and Papp-A KO diabetic (grade 1) mice.

Article Snippet: PAPP-A immunohistochemistry human kidney Fresh-frozen normal and diabetic kidney sections were obtained from AMSBIO (Abingdon, UK).

Techniques: Immunohistochemistry, Staining

Journal: Nature methods

Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples

doi: 10.1038/nmeth.2483

Figure Lengend Snippet: Shown are key metrics for RNase-H (orange), Ribo-Zero (pink) and total (black) libraries from pancreas and FFPE kidney RNA. ( a ) Percent of rRNA mapping reads; ( b ) Proportion of reads mapping to exons (solid), introns (hatched), and intergenic (white) regions; ( c ) mean coefficient of variation ( y axis) for the top 1,000 expressed transcripts in each library ( x axis); ( d ) Pearson correlation coefficient between each library and a control Total library.

Article Snippet: For human kidney FFPE tissue sections (Cybrdi), we isolated total RNA with the MasterPure RNA Purification Kit (Epicentre) following the manufacturer’s instructions with these modifications.

Techniques: Control

Journal: Cancer Science

Article Title: Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10

doi: 10.1111/j.1349-7006.2007.00701.x

Figure Lengend Snippet: Immunohistochemical staining analyses of synovial sarcoma and normal human tissue sections using MAb 92‐13; (a,b) synovial sarcoma tissue sections (c) colon, (d) heart, (e) lung, (f) liver, (g) kidney and (h) placenta. Original magnification ×100. Bar, 50 µm.

Article Snippet: For immunohistochemical staining analysis, 4‐µm sections of paraffin‐embedded normal adult human tissues (lung, heart, liver, kidney, colon and placenta) (BioChain, Hayward, CA, USA) and surgical synovial sarcoma specimens were deparaffinized and processed for antigen retrieval in Target Retrieval Solution (pH 9) (DAKO Cytomation, Carpinteria, CA, USA) at 125°C for 30 s. After treatment with peroxidase blocking reagent (DAKO Cytomation) for 10 min, followed by treatment with protein blocking serum‐free (DAKO Cytomation) for 30 min, slides were incubated with 10 µg/mL MAb 92‐13.

Techniques: Immunohistochemical staining, Staining

Journal: Aging (Albany NY)

Article Title: Tumor necrosis factor superfamily 14 is critical for the development of renal fibrosis

doi: 10.18632/aging.104151

Figure Lengend Snippet: Increased TNFSF14 expression in patients with fibrotic nephropathy. ( A ) The expression of TNFSF14 in human kidney sections from healthy control, CKD patients were detected by immunohistochemistry (upper lane, original magnification×200; lower lane, original magnification×400). ( B ) The expression of TNFSF14 receptors (HVEM and LTβR) in biopsied human kidney specimens from patients with CKD were detected by immunohistochemistry. (upper lane, original magnification×200; lower lane, original magnification×400). Nontumoral kidney tissue from patients with renal cell carcinoma was used as healthy control. ( C ) TNFSF14 levels in serum from healthy controls (n = 8) and patients with CKD (n = 16) were measured by ELISA. The data were representative of the results of three independent experiments. Values are represented as mean ± SEM. * P < 0.05.

Article Snippet: Optimal cutting temperature compound (O.C.T, Sakura Finetek, USA)-embedded human kidney biopsy sections (4-μm) were prepared as described previously [ ].

Techniques: Expressing, Control, Immunohistochemistry, Enzyme-linked Immunosorbent Assay